Becton Dickinson LSR II
The Center now maintains a dedicated flow cytometry center based around the BD LSR II cytometer in the Kazura Lab area, BRB 4th Floor SW Labs. Paula Embury and Rhonda Kimmel will coordinate scheduling, training and maintenance of this machine and can be reached at 368-4886, or pbe@case.edu.
There is an on-line calendar to coordinate the flow cytometer schedule at the following URL:
http://www.my.calendars.net/cghd_lsrii/
Please see her for your initial training and any Diva software or instrument issues. We intend to have the flow available during "normal business hours": M-F from 9am - 5pm. Please include your full name, e-mail, and phone number in your on-line reservation. Thanks for your cooperation!
Additional Information about the LSR II
LASER |
FLUOROCHROME |
DETECTOR |
Mirror |
FILTER |
OTHER INFO |
| 488nm Blue |
PE-Cy7 |
A |
735 LP |
780/60 |
|
| 488nm Blue |
PerCP |
B |
635 LP |
670/14 |
PerCP-Cy5.5 |
| 488nm Blue |
Alexa 610 |
C |
600 LP |
610/20 |
PE-Texas Red |
| 488nm Blue |
PE |
D |
550 LP |
575/26 |
GFP |
| 488nm Blue |
FITC |
E |
505 LP |
530/30 |
Alexa 488 |
| 488nm Blue |
Side Scatter |
F |
|
488/10 |
Side Scatter |
| |
|
|
|
|
|
| 633nm Red |
APC-Alexa 750 |
A |
735 LP |
780/60 |
APC-Cy7 |
| 633nm Red |
Alexa 700 |
B |
685 LP |
730/45 |
|
| 633nm Red |
APC |
C |
|
660/20 |
Alexa Fluor 660 |
Online Protocols for using the LSR II
Coming soon! |
