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David R. Kaplan, M.D., Ph.D.ProfessorMailing Address: fax: (216) 368-0494 email: David.Kaplan@Case.edu |
Biography
Dr. David Kaplan graduated with an A.B. in Biology from the University of Chicago in 1974. He later went on
to earn a Ph.D. in Immunology and a M.D. in Medicine. His residency was in Pathology at Washington
University from 1980 until 1984, after which Dr. Kaplan was a Research Fellow. In 1984 he came to
Case’s Department of Pathology as an Assistant Professor. From 1984 to 2000, Dr. Kaplan directed the
Immunology Laboratories at University Hospitals of Cleveland. Presently, he is a Professor of Pathology.
Research
Flow cytometric analysis is a powerful technique for the single cell assessment of molecules expressed at
low levels. The major deficiency of flow cytometry has been its relative insensitivity. Only
molecules expressed in abundance have been readily observed. We have developed an enzymatic
amplification procedure for flow cytometry that increases the fluorescence signal between 10 and 100
fold thereby producing a significant enhancement in the resolving power of the technique. Using this
technique we have been able to detect the presence of molecules that could not be observed by the
standard procedure. With enzymatic amplification staining, we have significantly enhanced the sensitivity
of flow cytometric analysis for surface receptors and a variety of intracellular analytes such as
cyclin molecules, phosphoantigens, transcription factors, mitochondrial molecules, apoptosis mediators,
and RNA. Enzymatic amplification staining gives a resolving capability that is comparable to the sensitivity
of western analysis.
I have been interested in the reform of peer review both for the evaluation of manuscripts for publication by journals and for the assessment of grant applications for funding. I have proposed several reforms that I am working on implementing.
Publications
Kaplan D, Smith D (2000). Enzymatic amplification staining for flow cytometric analysis of cell
surface molecules. Cytometry 40, 81-85.
Kaplan, D, H Meyerson, K Lewandowska. High resolution immunophenotypic analysis of chronic lymphocytic leukemia cells by enzymatic amplification staining (EAS). Amer J. Clin. Path. 116:429-36, 2001.
Kaplan, D, D Smith, H Meyerson, N Pecora, K Lewandowska. CD5 expression by B lymphocytes and its regulation upon Epstein-Barr Virus transformation. Proc. Natl. Acad. Sci. USA. 98:13850-13853, 2001.
Kaplan, D, W Husel, K Lewandowska. CD34 expression on platelets. Platelets. 14:83-87, 2003.
Kaplan, D. Enzymatic amplification staining for cell surface antigens. Current protocols in cytometry. Wiley. New York. Pp 6.14.1-6.14.11, 2003.
Kaplan, D. Enzymatic amplification staining for single cell analysis: applied to in situ hybridization. J Immunol. Methods 283:1-7, 2003.
Kaplan, D, H Meyerson, W Husel, K Lewandowska, G MacLennan. D cyclins in lymphocytes. Cytometry 63A:1-9, 2005.
Meyerson, H, G MacLennan, W Husel, W Tse, H Lazarus, D Kaplan. D cyclins in CD5+ B cell lymphoproliferative disorders: cyclin D1 and cyclin D2 identify diagnostic groups and cyclin D1 correlates with ZAP-70 expression in chronic lymphocytic leukemia. Amer J Clin Path In press, 2005.
Kaplan, D. How to fix peer review. The Scientist 19(11):10, 2005.
Kaplan, D. Reform of Peer Review at NIH The Scientist 19(17):10, 2005.