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| กก |
Characterization of the cholesterol content of cell plasma membrane
The aim of this research is to develop electrochemical methodology that will investigate the cholesterol content of cell plasma membranes. The ability to evaluate the dynamics of intracellular cholesterol trafficking to and from the plasma membrane would allow characterization of pathways governing cholesterol homeostasis and, in particular, of the initial steps in atherogenesis. Our group has established a procedure for fabrication of an electrode-supported lipid bilayer membrane that allows immobilization of cholesterol oxidase, which could be employed to detect the cholesterol contained in the plasma membrane of a single cell. The steady-state electrode response observed appears to correlate with the cholesterol content of plasma membrane.
Model of electrode-supported lipid bilayer membrane containing cholesterol oxidase
for single cell cholesterol analysis
DNA Binding of Platinum Based Anticancer Drugs
The aim of this research is to probe, in real time, the contributions of electrostatic and hydrogen bonding interactions to the sequence specificity of platinum binding to DNA and RNA. The binding rates are being studied using a quartz crystal microbalance (QCM) modified with a target sequence. This approach is also well suited for evaluating the recognition of DNA-platinum adducts by biomolecules possibly involved in repair of damaged DNA and drug resistance.