Optional Homework 6.
The following data were obtained for the binding of the drug fluoxetene to a protein depressin.
[B] x 106 M Bbound x 106 M
|
1 |
1.25 |
|
3 |
2.81 |
|
5 |
3.75 |
|
7 |
4.38 |
|
9 |
4.82 |
|
11 |
5.16 |
|
13 |
5.42 |
|
15 |
5.63 |
|
17 |
5.80 |
|
19 |
5.94 |
|
21 |
6.06 |
|
23 |
6.16 |
|
25 |
6.25 |
|
27 |
6.33 |
|
29 |
6.40 |
|
31 |
6.46 |
|
33 |
6.51 |
|
35 |
6.56 |
|
37 |
6.61 |
|
39 |
6.65 |
|
41 |
6.68 |
|
|
|
The concentration of the protein used in the binding experiment was 2.5 x 10-6 M.
1. Determine whether the binding is simple or complex (Answer: simple binding).
2. Determine the number of binding sites on depressin for fluoxetene (Answer: 3 sites per molecule)
3. Determine the intrinsic dissociation constant for these sites (Answer: 5.0 x 10-6 M)
The analyses of the data should be done graphically using the direct plot, double reciprocal or Scatchard plots.