CF Ibuprofen Lab: Brief Method Description
The method used for the determination of ibuprofen in plasma was previously developed and has been used in other ibuprofen studies. The determination utilized high-performance liquid chromatography (HPLC) to detect and quantify ibuprofen. The ibuprofen and added internal standard ibufenac contained within plasma were extracted with hexane / isopropanol. The residue was then injected into the HPLC and the resulting chromatographic peaks were detected with a UV detector at 220 nm. Linear standard curves were calculated based on the chromatographic peak areas of know amounts of ibuprofen compared to peak areas of the added internal standard, ibufenac. The ibuprofen concentrations in patient plasma samples were interpolated using these standard curves.
Minkler, P.E., Hoppel, C.L., "Determination of ibuprofen in human plasma by high performance liquid chromatography", (1988) J. Chromatogr. 428, 388-394.
August 22, 1997.
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